WebbFor secretion of fusion proteins, the open reading frames were cloned into the Escherichia coli-Bacillus megaterium shuttle vector pHIS1525. After transformation of the respective plasmids into Bacillus megaterium protoplasts, the recombinant genes were successfully expressed and the proteins were secreted into the growth medium. WebbPlasmids pHIS1522/pHIS1525 both contain the strong xylA promoter (PxylA)originating from Bacillus megaterium. Transcription from this promoter is xyloseinducible (after xylose addition, the xylose repressor coded by the xylR gene onthe plasmids is released from PxylA and transcription is initiated).
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Webb23 jan. 2024 · FISH. 关注. 直接把载体告诉测序公司,如果载体比较常见,公司直接有现成的测序引物提供。. 只需提供质粒或菌液给到公司就行。. 如果载体不常见也可以自己设计 … Webb应《网络安全法》要求,自2024年10月1日起,未进行实名认证将不得使用互联网跟帖服务。为保障您的帐号能够正常使用,请尽快对帐号进行手机号验证,感谢您的理解与支持! scottsdale softball schedule
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Webb2 apr. 2012 · The aim of this research was to express the β-xylosidase gene in the pHIS1525/ Bacillus megaterium MS941 system. The xyl genewas amplified from pTP510 and cloned into pHIS1525 in E. coli ... WebbContext 1. ... pHIS1525 is a shuttle vector for E. co- li/B. megaterium. Plasmid pHIS1525 contains a sig- nal peptide for secretion of heterologous protein of interest into the … http://www.biovector.net/product/1077.html scottsdale silver 10 oz bars wholesale