Lamp2b-ha

Author: ntrr

August undefined, 2024

TīmeklispcDNA Flag Lamp2b-HA质粒价格1200元，pcDNA Flag Lamp2b-HA(爱迪基因#71292 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为氨苄青霉素，质粒大 … Tīmeklis2024. gada 23. maijs · To produce CTL-Exo and CTP-Exo, HEK 293 cells were transfected with FLAG-Lamp2b-HA or CTP-FLAG-Lamp2b-HA plasmids, respectively. To monitor plasmid expression and exosome uptake, mCherry-CD81 plasmids were co-transfected with Lamp2b plasmids (Fig. 1 B). CTL-Exo and CTP-Exo were purified …

pcDNA GNSTM-3-Flag-10-Lamp2b-HA - biofeng.com

Tīmeklis2024. gada 4. maijs · To produce sEVs RVG-siRNA, a recombinant vector, pcDNA-RVG10-Lamp2b-HA, was constructed and transfected into human embryonic kidney (HEK) 293T cells.The secreted sEVs were isolated from the culture supernatants by differential ultracentrifugation, as previously described. 16 The purified RVG-modified … TīmeklisLAMP2 ( a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110) Promoter CMV Tag / Fusion Protein HA (C terminal on insert) Cloning Information Cloning method … new philadelphia texas roadhouse

pcDNA GNSTM-3-10-Lamp2b-HA _载体质粒_质粒载体网-质粒,微 …

TīmeklispcDNA GNSTM-3-RVG-10-Lamp2b-HA (Plasmid #71294) Print Enlarge View all sequences Purpose Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, … TīmeklispMEH31 Lamp2b HA: Lamp2b (Homo sapiens) Leonard Add to Cart 168209: pMEH60 Lamp2b MS2 HA: Lamp2b (Homo sapiens) Leonard Add to Cart 183645: pBoBi … Tīmeklis2024. gada 10. janv. · In this study, a TfR-targeting exosome was produced by creation of a fusion protein of T7 peptide and Lamp2b ( Fig. 1 A ). After systemic … new philadelphia school system

Supplementary Figure 1. Western blot assay with anti-HA …

Tīmeklis2024. gada 7. janv. · pcDNA GNSTM-3-RVG-10-Lamp2b-HA. catalog : 71294. more info or order : Addgene product webpage. citations: 6. Reference; Li B, Chen X, Qiu W, Zhao R, Duan J, Zhang S, et al. Synchronous Disintegration of Ferroptosis Defense Axis via Engineered Exosome-Conjugated Magnetic Nanoparticles for Glioblastoma … Tīmeklis2024. gada 20. jūl. · The construction of pRBP-Lamp2b-HA has been previously reported. 36 HA is a commonly used epitope tag, which does not interfere with the … intro to typing crossword clueTīmeklisHuman Lamp2b cDNA clone 3543019 was purchased from Open Biosystems (now sold by Dharmacon) and inserted into pcDNA3.1+ Hygro (Clontech). A C-terminal GSG spacer and an HA peptide tag (YPYDVPDYA) were added by … intro to twilight zone

"Tīmeklis一种基于Lamp2b的融合蛋白可用于在表面显示狂犬病病毒糖蛋白（RVG），当这些胞外体被系统地给予时，表面展示的RVG导致这些胞外体在小鼠的神经元和大脑中积聚。去除Lamp2b RVG中的信号肽可显著降低RVG在工程化胞外体上的展示。其他用于融合蛋白外体表面显示的常用分子包括四跨膜蛋白（CD63、CD9、CD81）、糖基磷脂酰 … " - Lamp2b-ha

Lamp2b-ha

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TīmeklisLamp2b-HA and T7-Lamp2b-HA contained the HA tag as fusion proteins of Lamp2b. GAPDH was detected as an internal control. CD63 was detected as an exosome marker. 7KLV (OHFWURQLF6XSSOHPHQWDU\0DWHULDO (6, IRU1DQRVFDOH MRXUQDOLV 7KH5R\DO6RFLHW\RI&KHPLVWU\ Supplementary Figure 2. …

Tīmeklis2024. gada 24. dec. · Mutations in lysosomal-associated membrane protein 2 ( LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart failure through poorly defined mechanisms. Here, we identify the LAMP-2 isoform B (LAMP-2B) as required for autophagosome–lysosome fusion in human … TīmeklisSequences pcDNA GNSTM-3-RVG-10-Lamp2b-HA Sequences (3) Addgene Sequences: Partial (2) Depositing Scientist Sequences: Full (1) Full Sequences from …

Tīmeklis2024. gada 24. dec. · Mutations in lysosomal-associated membrane protein 2 ( LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart … TīmeklispMEH60 Lamp2b MS2 HA (Plasmid #168209) Print Enlarge View all sequences Purpose Mammalian expression of lysosome-associated membrane protein 2b fused …

TīmeklisDownload scientific diagram Identification of CBD-Lamp2b-HA encoding in the stable cell line. (A) The relative mRNA expression of fusion protein in negative control and …

TīmeklisPlasmid pMEH31 Lamp2b HA from Dr. Joshua Leonard's lab contains the insert Lamp2b and is published in J Extracell Vesicles. 2016 May 13;5:31027. doi: 10.3402/jev.v5.31027. eCollection 2016. This plasmid is available through Addgene. new philadelphia sp 15Tīmeklis2024. gada 10. febr. · Plasmid DNA (pDNA), which expressed RBP-Lamp2b-HA, was transfected to 293 T cells and a stable cell line with pDNA was established by selection with hygromycin. Exosomes from the cells were purified and the presence of RBP-Lamp2b-HA on the exosomes was confirmed by a western blot assay with anti-HA … new philadelphia staplesTīmeklisGoat Anti Lamp2b, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … intro to trigonometry pptTīmeklis2015. gada 3. marts · Exosomes and cells were incubated for 2 h at 37 °C, as described (11). Cells were then washed with PBS and harvested for flow cytometry. Flow … new philadelphia restaurants 2021TīmeklisHA (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site NheI (destroyed during cloning) 3′ cloning site BamHI (destroyed during … intro to tubthumpingTīmeklis2024. gada 10. nov. · To produce sEVs RVG -siRNA, a recombinant vector, pcDNA-RVG10-Lamp2b-HA, was constructed and transfected into human embryonic kidney (HEK) 293T cells. The secreted sEVs were isolated from the culture supernatants by differential ultracentrifugation, as previously described. 16 new philadelphia rotary clubTīmeklis2024. gada 1. dec. · The pcDNA GNSTM-3-Flag-10-Lamp2b-HA plasmid (Flag-Lamp2b) was obtained from AddGene (Cambridge, MA; AddGene plasmid #71293, originally provided by Joshua Leonard [21]). For construction of the ABP-Lamp2b fusion plasmid (pcDNA GNSTM-3-ABP-10-Lamp2b-HA), the cDNA fragment encoding … intro to two column proofs