Hispur ni-nta superflow agarose
WebThermo Scientific HisPur Ni-NTA Superflow Agarose is a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni +2 ) designed... Thermo …
Hispur ni-nta superflow agarose
Did you know?
WebNi-NTA agarose is equilibrated in Buffer 3B and a 50% slurry in this buffer is prepared. This slurry (1 ml/2 liter cell culture) is added to the cell lysate and the sample is mixed for 30 min at 4° using a test tube rocker. This mixture is then sequentially transferred to a 5 ml polypropylene column (Qiagen). Web12 mag 2024 · The reuse of Ni-NTA Agarose and Ni-NTA Superflow resins depends on the nature of the sample and should only be performed with identical recombinant …
WebHisPur Ni-NTA Superflow Agaroseは高度にクロスリンクされた高耐久性レジンで、中規模または大規模のFPLC精製に使用される流量で圧縮しません。 このレジンはさまざ … WebHisPur™ Ni-NTA Superflow Agarose. WARNING: Cancer – www.P65Warnings.ca.gov. For Research Use Only. Not for use in diagnostic procedures. Specifications. Capacity …
WebHisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications. Highlights: … Web26 nov 2024 · The isolation of recombinant His-tagged α-MMC protein was achieved by Ni-NTA affinity chromatography and the purity was shown in 12% SDS-PAGE electrophoresis ( Figure 1 A). In our expression system, approximate 50 mg recombinant protein could be purified from 1 L of Rosetta culture.
WebPierce™ HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni⁺²) used for FPLC purification of poly-histidine …
WebProduct Size Cat. No. Resin only HisPur Ni-NTA Agarose Resin 10 mL 88221 100 mL 88222 500 mL 88223 HisPur Ni-NTA Superflow Agarose Resin 10 mL 25214 50 mL 25215 250 mL 25216 1 L 25217 Product Size Cat. No. Resin only HisPur Cobalt Agarose Resin 10 mL 89964 100 mL 89965 500 mL 89966 HisPur Cobalt Superflow Agarose … picture of taye diggs sonWeb21 dic 2024 · HisPur Ni-NTA Superflow Agarose (Thermo Fisher Scientific) for 1h at 4ºC. Resin bound with S protein was separated from unbound proteins and medium using a gravity flow column, followed by 30 column volume wash with wash buffer (65 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole, pH 7.5). 4. 9 picture of tawny owlWebHisPur Ni-NTA Superflow Agarose Section 2. Hazards identification Prevention :Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. Use personal protective equipment as required. Wear protective gloves. Wear eye or face protection. In case of inadequate ventilation wear respiratory ... picture of taxi in sri lankaWebHisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications. Highlights: … picture of tautogWebThermo Scientific HisPur Ni-NTA Superflow Agarose is a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2) designed for FPLC … top gear season 15 episode 6 watch onlineWebRelated products Product Cat. no. HisPur™ Ni-NTA Resin 88221 HisPur™ Cobalt Resin 89964 HisPur™ Ni-NTA Superflow Agarose 25214 HisPur™ Cobalt Superflow Agarose 25228 HisPur™ Ni-NTA Chromatography Cartridges 90098 Halt™ Protease Inhibitor Cocktail, EDTA-free (100X) 78425 Slide-A-Lyzer™ Dialysis Cassettes Kit 66382 Zeba™ … top gear season 15 episode 1Web24 mag 2014 · 25217 HisPur Ni-NTA Superflow Agarose, 1000mL settled resin. Binding Capacity: ≥ 60mg/mL of settled resin for a 27kDa 6xHis-tagged protein from a bacterial. … picture of taylor cole