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WebMay 17, 2013 · Figure 1: Choose FRAP wizard. At the top of the wizard interface, the working steps are displayed as buttons: “Overview”, “Set Up”, “Bleach”, “Time course”, “Evaluation” (Figure 2). The overview gives a description of the wizard and explains what to do in each step. Figure 2: FRAP wizard overview. Fluorescence recovery after photobleaching (FRAP) is a method for determining the kinetics of diffusion through tissue or cells. It is capable of quantifying the two-dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells. This technique is very useful in biological studies of cell membrane diffusion and protein binding. In a… cycling helmet baseball cap
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